ISOLASI DAN IDENTIFIKASI SENYAWA AKTIF
LARVASIDA DARI BIJI MIMBA (Azadirachta indika A. Juss) TERHADAP LARVA
NYAMUK DEMAM BERDARAH (Aedes aegypti)
| Article Info | ABSTRACT | |
| Published date: 13 Nov 2012 |
Isolation and
identification of larvicidal active compounds towards Aedes aegypti from
Mimba seed havebeen conducted. One kilogram dry powder of Mimba seed
was extracted with ethanol at room temperature.Evaporation of ethanol
gave 30 g of crude ethanol extract which showed activity against Aedes
aegypti (LC50282.29). This extract was dissolved into methanol-water
(7:3) and was then partitioned with n-hexane, chloroformand ethyl
acetate respectively. The three extracts obtained i.e. n-hexane,
chloroform and ethyl acetate were showedtheir activity against Aedes
aegypti in which the-n-hexane extract was the most active with LC50 of
143.97.Therefore the n-hexane extract was further purified using silica
gel column chromatography with chloroform:nhexane(9:1) as eluent. Three
fractions was obtained i.e. F1, F2 and F3 and they were all active
against Aedes aegyptiwith LC50 78.45, LC50 113.54 and LC50 58.70
respectively. It can be seen that F3 was the most active fractions
butfrom TLC result, F1 showed the relatively pure compounds since it
only gave one spot. Therefore F1 was furtheridentified using
pyhtochemical testing, Uv-Vis, infrared and GC-MS. It was found that the
larvicidal activecompounds F1 was belong to carboxylic acids
groups,with l max 290.1 having functional groups such as
methyl,methylene and carbonil. Identification using GC-MS indicated that
the larvicidal active compounds F1 was assumedto be a combination of 7
compounds derived from carboxylic acids including hexa-decanoic acid,
etil-hexadecanoate,oleic acid, etyl-oleate ester, octadecanoic acid,
etyl-octadecanoate and dioctyl-hexadioate.
|
Arda Dinata adalah Peneliti Kesehatan dan Penulis Buku "BERSAHABAT DENGAN NYAMUK: Jurus Jitu Atasi Penyakit Bersumber Nyamuk."
'new, bersahabat dengan nyamuk, nyamuk, umur nyamuk, morfologi nyamuk, klasifikasi nyamuk, nyamuk aedes aegypti, nyamuk demam berdarah, nyamuk anopheles, nyamuk chikungunya, nyamuk malaria, nyamuk kaki gajah, filariasis, je, penyakit nyamuk, penyakit nyamuk cikungunya, pengertian nyamuk, hindari penyakit nyamuk, demam berdarah nyamuk, nyamuk baru, reperensi penyakit nyamuk, akibat nyamuk, obat nyamuk, nyamuk blog, mosquito, anti mosquito, mosquito trap, mosquito sound, jurnal nyamuk, jurnal nyamuk aedes aegypti, jurnal pengendalian nyamuk, jurnal nyamuk cuex, nyamuk lingkungan, jurnal pengendalian nyamuk, nyamuk psn, jurnal nyamuk search, jurnal larva, aspirator, aspirator nyamuk, insect aspirator, vektor nyamuk, vektor nyamuk culex, struktur vektor nyamuk, vektor nyamuk anopheles, jurnal pengendalian vektor nyamuk, vektor nyamuk filariasis, pengertian vektor nyamuk, artikel vektor nyamuk, contoh vektor nyamuk, buku nyamuk, anatomi nyamuk, tanaman pengusir nyamuk, tanaman pengusir nyamuk dalam rumah, tanaman pengusir nyamuk blog, tanaman hias pengusir nyamuk, tanaman pengusir nyamuk paling ampuh, jual tanaman pengusir nyamuk, tanaman pengusir nyamuk alami, herbal, tompen, tanaman obat, lavender, zodia, wisata ilmiah nyamuk, musium nyamuk, insektarium nyamuk, tompen nyamuk, darah nyamuk'
