Penggandaan Skala Produksi Bioinsektisida Bacillus thuringiensis var. israelensis untuk Membasmi Jentik Nyamuk Aedes aegypti
| Article Info | ABSTRACT | |
| Published date: 18 Mar 2013 |
The objective of this
research is to study the scaling up of bionsecticide production from
Bacillus thuringiensisvar. israelensisusing onggok (a cassava
by-product) as a carbon source. The insecticide produced was used to
eradicate Aedes aegypti larvae. The product was a crystal protein
produced during bacterial sporulation. Scaling up from laboratory to
pilot plant scale was done using two methods, i.e. constant agitation
power per unit volume (Pg/V) and constant oxygen transfer coefficient
(kLa). The results showed that yield of product per substrate (Yp/s) of
Pg/V based product with the value of 3.52 ± 0.02 spora per gram
substrates was higher than Yp/s of kLa based product with the value of
2.96 spora per gram substrate. Logarithmic value of viable spore count
(log of VSC) was also higher, i.e. 7.23 ± 0.30 for Pg/V based product as
compared to 7.17 ± 0.20 for kLa based product. Substrate efficiency was
also higher in Pg/V based (92.47%) than kLa based (64.87%). LC50 of
Pg/V based product was lower (0.49 μg/ml) meaning that it was more toxic
than kLa based product (0.62 μg/ml). Amino acid content of Pg/V based
product was also higher than kLa based product. Constant Pg/V method was
suggested as a based on the scaling up of bioinsecticide production of
B. thuringiensis israe/ensison industrial scale.
Keywords:
bioinsecticide, Bacillus thuringiensisvar. israelensis, kLa, Pg/V, LC50,
viable spore count
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Arda Dinata adalah Peneliti Kesehatan dan Penulis Buku "BERSAHABAT DENGAN NYAMUK: Jurus Jitu Atasi Penyakit Bersumber Nyamuk."
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