Background: Dengue haemorhagic Fever (DHF) is an infectious disease caused by dengue virus and transmited through the bite of a mosquito Aedes aegypti. Spreading of DHF influenced by environmental factors and practice of breading place eradication. Work area of public health service of Srondol covered endemic area of DHF. Objective: To determine the realtionship between environment factors and eradication of mosquito breeding practices in families with incidence of DHF in the working area of public health service of Srondol Banyumanik sub district Semarang City. Method: This was an explanatory research with case control approach. The sample of (case) was all patiens with DHF patient that recorded in Public health service of Srondol from all health service unit between January to April 2010 (47 cases), and the sample of (control) was the people around the patient who do not suffered DHF with radius of 100 meters, between January to Juni 2010, which equated their ages, genders, and nutritional status (47 respondents). The dependent variable was incidence of DHF and the independent variable was the breeding place both indoor and outdoor, resting place both indoor and outdoor, lighting air humidity of room, the habit of closing the water reservoirs, the habit to draining water reservoirs and habit to removing secondhand goods. These data analyzed by Chi Square test. Results: There were realtionship between breeding place indoors (p=0,048), resting place outside the home (p=0,035), custom exhaust water reservoirs (p=0,036), and lighting the room (p=0,013) with incidence of DHF and not the relationship between breeding place outdoors (p=0,096) resting plce indoors (p=0,059), customary closing water reservoirs (p=0,062), habit of burying the former goods (p=0,223), and humidity of the room (p=0,483) with incidence of DHF. Cloncusions: The environmental factors and mosquito breeding practices was the relationship with incidence of DHF are : Breeding plce inside house, resting place outside house, habit to drain saving waters container, room lighting. Keywords: Environmental factors, Practices and mosquito eradication, DHF.